Catalase Write Up

This experiment was performed to determine the factors that positively bring catalase answer rates in the break waste of total heat peroxide. Catalase enzyme employment was measured through its break belt down of hydrogen peroxide into piddle and oxygen through the displacement of piddle. We compared the decomposition rates in samples with vary pH directs of 2-10. The samples with the most neutral ph level decomposed hydrogen peroxide the quickest and most efficiently. Hypothesis Catalase is a fast working enzyme that breaks down hydrogen peroxide into water and liquid. dictionary. com) Many factors affect the break down of hydrogen peroxide, one of the main factors that relates to the human personify is pH levels. With the strawman of strong acids or strong bases the production rate of oxygen gas will increase the rate of reaction. As quoted from allsciencefair. com The optimal pH prototype is about 7-8 (physiological pH of most cells), but a few enzymes stick out wo rk at extreme pH, such as protease enzymes in animal stomachs, which have an optimum of pH 1. This shows that for the human body catalase call for to be able to function properly at extreme pH levels, proving where our hypothesis was drawn from. http//www. all-science-fair-projects. com/science_fair_projects/105/901/69adbb37c02f0fb4f8b674fbae189d9f. hypertext mark-up language Procedure Record the pH level of the mix between catalase and the acid/base Dip the circles of perk up paper into the mixture and place on top of the inside of the crank bottle. Add 5mL of hydrogen peroxide into the glass bottle ensuring that none touches the open or the filter paper.Place the bottle in the water tray and slide the nossel chthonian the graduated cylinder full of water. Flip the bottle so the hydrogen peroxide mixes with the catalase filter paper. Measure the production of oxygen gas all(prenominal) 5 seconds for 1 minute. Repeat for the remaining pH levels. Materials -Sulphuric Acid (0. 05M) -Potassium hydroxide (0. 05M and 0. 5M) -Catalase -water tray -3 250mL beakers -pH paper -3 eye droppers -2 graduated Cylinders -Dissection probe -filter paper -Hole Punch -Water -Distilled water -square glass container with nossel -Safety glassesThrough the results that were gained in our experiment the information proves that as pH levels move towards their extremes, the rate of oxygen becomed decreases severely. As you can take to in our sixth trial on day 2 the pH was recorded at f 7 with a rate of reaction of INSERT HERE indicating to us our middle value to compare opposite data to. When looking at the trends of trial 1 which is a pH of 5 you can see that the rate decreases as it becomes more acid, and then when comparing to trial 4 with a pH of 2 in that respect is no oxygen gas being produced.Similarly when the solution becomes more base the rate decreases as we can see in trial twain which yields a pH of 8. As the curve of best fit demonstrates as the pH increa ses or decreases from the neutral of 7 the reaction rate onrushs 0. In the human body all organs produce catalase but the sterling(prenominal) quantity of catalase is found in the liver. In end this experiment and analyzing the results it is noticeable that for the human body to have its greatest rate of decomposition of hydrogen peroxide, a neutral environment is a necessity.If the human body was to suffer from a case of acidosis where the body became to acidic it would negatively affect the decomposition of hydrogen peroxide by diminish do the process. Hydrogen Peroxide is created by many enzymes in our body that break down fatty acids and amino acids. When the hydrogen peroxide is created its unbroken in peroxisomes as it can be damaging. Peroxisomes are also where our catalase is stored allowing for the unproblematic break down of the hydrogen peroxide into water and oxygen. http//www. madsci. org/posts/archives/1999-09/938519528. Bc. r. html http//www. scribd. com/doc/908 88398/Catalase-Blood-Hydrogen-Peroxide